Capillary Electrophoresis - Mass Spectrometry for Proteomics and Metabolomics Principles and Applications

A powerful and essential resource for researchers with an interest in CE-MS

In Capillary Electrophoresis Mass Spectrometry for Proteomics and Metabolomics: Principles and Applications, a team of distinguished researchers delivers a comprehensive overview of bioanalytical capillary electrophoresis coupled to mass spectrometry (CE-MS). The book explains foundational principles, technology as well the strategies and techniques used in data analysis for metabolic and proteomic studies. It also provides a global overview of recent developments and advances for improving CE-MS sensitivity and reproducibility.

An essential handbook for everyone performing metabolomic and proteomic analysis, the information provided here will assist researchers in tapping into the full potential of this technique to answer biological and clinical questions.

Readers will also find:

• A thorough introduction to the principles of capillary electrophoresis, including its fundamentals, CE separation modes, capillary coatings, and the fundamentals of mass spectrometry
• In-depth examinations of technological developments in capillary electrophoresis, including sample preparation, online preconcentration, detection sensitivity, and metabolic coverage
• Comprehensive discussions of metabolomic studies, including their biomedical and clinical applications
• Recent advances in proteomics, including top-down and bottom-up approaches

Perfect for analytical and clinical chemists, Capillary Electrophoresis Mass Spectrometry for Proteomics and Metabolomics: Principles and Applications will also earn a place in the libraries of biochemists, molecular biologists, and other molecular life scientists.

Rawi Ramautar, PhD, is Principal Investigator at the Leiden Academic Centre for Drug Research, Leiden University (The Netherlands). He is also the editor of the Microchemical Journal.

David Chen, PhD, is Full Professor of Chemistry in the Faculty of Science at the University of British Columbia (Columbia) and an Associate Member in the Department of Anesthesiology, Pharmacology & Therapeutics.

PART I
PRINCIPLES

1. Fundamentals of Capillary Electrophoresis
2. CE Separation Modes
3. Capillary coatings
4. Fundamentals of Mass spectrometry
5. CE-MS interfaces (note: mass analyzers will be discussed here)
6. Data analysis strategies

PART II
TECHNOLOGICAL DEVELOPMENTS

1. Sample preparation and online preconcentration
2. Detection sensitivity and metabolic coverage
3. Throughput

PART III
COMPREHENSIVE OVERVIEW OF METABOLOMIC STUDIES

1. Biomedical and clinical applications
2. Plant and microbial applications
3. Environmental and food applications

PART IV OVERVIEW OF PROTEOMICS

1.Top-down proteomics
2.Bottom-up proteomics