Known worldwide as the standard introductory text to this important and exciting area of study, Gene Cloning and DNA Analysis: An Introduction, 8th Edition preserves the tradition of excellence created by previous editions. Comprehensive and authoritative, the book explores all of the topics crucial to an understanding of gene cloning in an approachable way. An easy-to-follow and user-friendly layout is presented in full-color throughout the volume, making it simple to absorb the clear and accessible material contained within.  

Gene Cloning and DNA Analysis: An Introduction, 8th Edition contains updated and extended coverage of gene editing strategies like CRISPR/Cas, rewritten chapters on DNA sequencing and genome studies, as well as new material on real-time PCR and typing of human disease mutations. Over 250 full-color illustrations are included to bring to life the comprehensive content. The book also covers topics like: 

• The strategies used by researchers and industry practitioners to assemble genome sequences 
• Next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes 
• Includes the use and application of gene editing strategies  
• Interbreeding between Neanderthals and Homo Sapiens  

Gene Cloning and DNA Analysis: An Introduction, 8th Edition is an invaluable introductory text for students in classes like genetics and genomics, molecular biology, biochemistry, immunology, and applied biology. It also belongs on the bookshelves of every professional who desires to improve their understanding of the basics of gene cloning or DNA analysis. 

T. A. Brown is Emeritus Professor of Biomolecular Archaeology in the Manchester Institute of Biotechnology at the University of Manchester in the United Kingdom. He has published several books on genetics, genomics and biochemistry as well as over 150 research papers.

Preface to the Eighth Edition

Part I The Basic Principles of Gene Cloning and DNA Analysis

1 Why Gene Cloning and DNA Analysis are Important

2 Vectors for Gene Cloning: Plasmids and Bacteriophages

3 Purification of DNA from Living Cells

4 Manipulation of Purified DNA

5 Introduction of DNA into Living Cells

6 Cloning Vectors for Escherichia coli

7 Cloning Vectors for Eukaryotes

8 How to Obtain a Clone of a Specific Gene

9 The Polymerase Chain Reaction

Part II The Applications of Gene Cloning and DNA Analysis in Research

10 Sequencing Genes and Genomes

11 Studying Gene Expression and Function

12 Studying Genomes

Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology

13 Studying Transcriptomes and Proteomes

14 Production of Protein from Cloned Genes

15 Gene Cloning and DNA Analysis in Medicine

16 Gene Cloning and DNA Analysis in Agriculture

17 Gene Cloning and DNA Analysis in Forensic Science and Archaeology

Glossary

Index