Preparative Centrifugation A Practical Approach

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Format: Paperback
Pub. Date: 1993-02-04
Publisher(s): IRL Press
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Summary

While the theoretical basis of centrifugal separations remains the same, recent years have seen dramatic changes in both the design of centrifuges and the range of people that use them. This book reflects these changing uses of preparative centrifuges by providing detailed protocols covering all of the different types of separation from DNA to cells. Guidelines are also given to help the reader devise new types of separation protocols. The book includes program source code for calculation and simulation programs that are invaluable for quantitative centrifugation methods. In addition, this volume contains extensive appendices of valuable data that are required by everyone using centrifuges as part of their research work. Preparative Centrifugation: A Practical Approach contains a wealth of guidelines, protocols, and practical advice that will be of direct use to experienced researchers and novices alike in virtually every area of biological research.

Table of Contents

Theoretical Aspects of Practical Centrifugation
Centrifuges and Rotors
Conditions for Density Gradient Separations
Centrifugal Methods for Characterizing Macromolecules and Their Interactions
Measurements of Sedimentation Coefficients
Isolation and Characterization of Membranes and Cell Organelles
Centrifugal Separations for Mammalian Cells
Separations in Zonal Rotors
Table of Contents provided by Publisher. All Rights Reserved.

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